The EpiQuik™ Global Tri-Methyl Histone H4-K20 Quantification Kit (Fluorometric) is a complete set of essential components, which enables the experimenter to fluorometrically measure global tri-methylation of histone H4-K20. The EpiQuik Global Tri-Methyl Histone H4-K20 Quantification Kit (Fluorometric) is suitable with using a variety of mammalian cells (human, mouse, etc.), including fresh and frozen tissues, and cultured adherent and suspension cells.

WHY CHOOSE THE EPIQUIK™ GLOBAL TRI-METHYLY HISTONE H4-K20 QUANTIFICATION KIT (FLUOROMETRIC)?

• Quick and efficient procedure, which can be finished within 2.5 hours.
• Innovative fluorometric assay without the need for radioactivity, electrophoresis, or chromatography.
• Specifically captures tri-methylated H4-K20 with the detection limit as low as 1 ng/well and detection range from 10 ng to 2 µg/well of histone extracts.
• The control is conveniently included for the quantification of tri-methylated H4-K20.
• Strip microplate format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ Global Tri-Methyl Histone H4-K20 Quantification Kit (Fluorometric) is designed for measuring global histone H4-K20 tri-methylation. In an assay with this kit, the tri-methylated histone H4 at lysine 20 is captured to strip wells coated with an anti-trimethyl H4-K20 antibody. The captured tri-methylated histone H4-K20 can then be detected with a detection antibody, followed by a fluorescent development reagent. The ratio of tri-methylated H4-K20 is proportional to the intensity of fluorescence. The absolute amount of tri-methylated H4-K20 can be quantitated by comparing to the standard control. 

SCHEMATIC PROCEDURE:

F1 (10X Wash Buffer)
F2 (Antibody Buffer)
F3 (Detecting Antibody, 1 mg/ml)*
F4 (Fluoro Developer)*
F5 (Fluoro Enhancer)*
F6 (Fluoro Dilution)
Standard Control (100 µg/ml)*
Signal Report Solution*
Signal Enhancer*
8-Well Sample Strips (with Frame)
8-Well Standard Control Strips
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]