The EpiQuik Global Mono-Methyl Histone H4-K20 Quantification Kit (Fluorometric) is a complete set of essential components, which enables the experimenter to measure mono-methylation of histone H4-K20. H4-K20 mono-methylation is involved in the maintenance of proper higher order structure of DNA and is consequently essential for chromosome condensation, it functions in gene silencing. The global H4-K20 mono-methylation can also be changed by inhibition or activation of HMTs, making quantitative detection of global mono-methyl histone H4-K20 useful in developing a better understanding of epigenetic regulation of gene activation/repression and for developing HMT-targeted drugs.

WHY CHOOSE THE EPIQUIK GLOBAL MONO-METHYL HISTONE H4-K20 QUANTIFICATION KIT (FLUOROMETRIC)?

• Quick and efficient procedure, which can be finished within 2.5 hours.
• Innovative fluorometric assay without the need for radioactivity, electrophoresis, or chromatography.
• Specifically captures mono-methylated H4-K20 with the detection limit as low as 1 ng/well and a detection range from 10 ng-2 µg/well of histone extracts.
• The control is conveniently included for the quantification of mono-methylated H4-K20.
• Strip microplate format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ Global Mono-Methyl Histone H4-K20 Quantification Kit (Fluorometric) is designed for measuring global histone H4-K20 mono-methylation. In an assay with this kit, the mono-methylated histone H4 at lysine 20 is captured to the strip wells coated with an anti-monomethyl H4-K20 antibody. The captured mono-methylated histone H4-K20 can then be detected with a detection antibody, followed by a fluorescent development reagent. The ratio of mono-methylated H4-K20 is proportional to the intensity of fluorescence. The absolute amount of mono-methylated H4-K20 can be quantitated by comparing to the standard control.

SCHEMATIC PROCEDURE:

F1 (10X Wash Buffer)
F2 (Antibody Buffer)
F3 (Detecting Antibody, 1 mg/ml)*
F4 (Fluoro Developer)*
F5 (Fluoro Enhancer)*
F6 (Fluoro Dilution)
Standard Control (100 µg/ml)*
Signal Report Solution*
Signal Enhancer*
8-Well Sample Strips (with Frame)
8-Well Standard Control Strips
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]