The EpiQuik™ Global Mono-Methyl Histone H3-K4 Quantification Kit (Fluorometric) is a convenient package of tools that allows the experimenter to specifically measure global mono-methylation of histone H3-K4 fluorometrically, using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

WHY CHOOSE THE EPIQUIK™ GLOBAL MONO-METHYL HISTONE H3-K4 QUANTIFICATION KIT (FLUOROMETRIC)?

• Quick and efficient procedure, which can be finished within 2.5 hours.
• Innovative fluorometric assay without the need for radioactivity, electrophoresis, and chromatography.
• Specifically captures mono-methyl H3-K4 with the detection limit as low as 0.4 ng/well and detection range from 20 ng-5 µg/well of histone extracts.
• The control is conveniently included for the quantification of mono-methylated H3-K4.
• Strip microplate format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ Global Mono-Methyl Histone H3-K4 Quantification Kit (Fluorometric) is designed for measuring global histone H3-K4 mono-methylation. In an assay with this kit, the mono-methylated histone H3 at lysine 4 is captured to the strip wells coated with an anti-mono-methyl H3-K4 antibody. The captured mono-methylated histone H3-K4 can then be detected with a labeled detection antibody, followed by a fluorescent development reagent. The ratio of mono-methylated H3-K4 is proportional to the intensity of fluorescence. The absolute amount of mono-methylated H3-K4 can be quantitated by comparing to the standard control.

SCHEMATIC PROCEDURE:
	Histone extracts were prepared from MDA-231 cells using the EpiQuik Total Histone Extraction Kit and the amount of monomethyl-H3-K4 was measured using the EpiQuik Mono-Methyl Histone H3-K4 Quantification kit (Fluorometric).

F1 (10X Wash Buffer)
F2 (Antibody Buffer)
F3 (Detection Antibody, 1 mg/ml)*
F4 (Fluoro Developer)*
F5 (Fluoro Enhancer)*
F6 (Fluoro Dilution)
Standard Control (100 µg/ml)*
8-Well Sample Strips (with Frame)
8-Well Standard Control Strips
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.