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Introduction to Epigenetics

Phospho Histone H3 (Thr11) Polyclonal Antibody

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Application: ELISA, IF, IHC, IP, WB
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
Application Guarantee: 6 months
Catalog No.SizePriceQty
A-4052-02525 µg $139.00 
A-4052-05050 µg $269.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

Background 
Histone H3 along with H2A, H2B and H4 is involved in the structure of chromatin in eukaryotic cells. Histone H3 can undergo several different types of epigenetic modifications that influence cellular processes. These modifications, including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation, occur on the N-terminal tail domains of histone H3, which results in remodeling of the nucleosome structure into an open conformation more accessible to transcription complexes. In most species, histone H3 is primarily phosphorylated at serine 10, serine 28, threonine 3 and threonine11. 

Concentration
1 mg/ml

Description
Rabbit polyclonal antibody raised against a synthetic peptide corresponding to the phosphorylated histone H3 at Thr11

Purification
Protein A purified

Specificity
Detects histone H3 only when phosphorylated at Thr11 in mouse, rat, and human. Broad species cross-reactivity predicted based on sequence homology

Isotype
IgG

Formulation 
PBS (pH 7.5), 150 mM NaCl, 30% glycerol

Storage
-20°C, stable for 1 year from the date of shipment. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance

Alternative Names
H3thr11P antibody

Application 
WB: 1:1000-1:2000, IF: 1:100-1:500, IHC: 1:100-1:500, ELISA: 1:1000-1:2000, IP: 2 μg/106 cells

Fig. 1. WB analysis of Phospho-Histone H3 (Thr11) Polyclonal Antibody with colcemid-treated HeLa cell lysates (A-4052).

 

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