Description
Mouse monoclonal antibody to 5-methylcytosine (5-mC), alternate name 5-methylcytidine, clone 33D3, ChIP-grade

Concentration
1 mg/ml

Specificity
Modified base 5-methylcytosine (5-mC), a broad range of species.

Isotype
IgG1

Formulation
10 mM phosphate buffer, 150 mM NaCl, pH 7.4

Storage
4°C, stable for 6 months from the date of shipment. For long-term storage, aliquot and store at -20°C. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Application
WB: 1:200-1:500; IHC: 1:100-1: 500, ELISA: 1:1000-1:2000; IP: 2 µg/10⁶ cells

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Ge, C. et. al. (March 2012). A simple colorimetric detection of DNA methylation. Analyst. Epub ahead of print. PubMed Abstract

Li, Y. et. al. (February 2012). IL-2 and GM-CSF are regulated by DNA demethylation during activation of T cells, B cells and macrophages. Biochem Biophys Res Commun. Epub ahead of print. PubMed Abstract

Barra, V. et. al. (February 2012). Bypass of cell cycle arrest induced by transient DNMT1 post-transcriptional silencing triggers aneuploidy in human cells. Cell Div. Epub ahead of print. PubMed Abstract

Zhang, Y. et. al. (October 2011). Effects of DNMT1 silencing on malignant phenotype and methylated gene expression in cervical cancer cells. J Exp Clin Cancer Res. 30(1):98. PubMed Astract

Aoi, Y. et. al. (October 2011). The involvement of DNA and histone methylation in the repression of IL-1ß-induced MCP-1 production by hypoxia. Biochem Biophys Res Commun. 414(1)252-8. PubMed Abstract

Li, L. et. al. (July 2011). Genome-wide DNA methylation patterns in IVF-conceived mice and their progeny: a putative model for ART-conceived humans. Reprod Toxicol. 32(1):98-105. PubMed Abstract

Liu, S. et. al. (March 2011). Effect of gonadotropins on dynamic events and global deoxyribonucleic acid methylation during in vitro maturation of oocytes: an animal model. Fertil Steril. 95(4): 1503-1506.e3. PubMed Abstract

Mandrioli, M. et. al. (January 2011). Composition and Epigenetic Markers of Heterochromatin in the Aphid Aphis nerii (Hemiptera: Aphididae). Cytogenet Genome Res. Epub ahead of print. PubMed Abstract

Yang, Y. et. al. (October 2010). Identification of methylated regions with peak search based on Poisson model from massively parallel methylated DNA immunoprecipitation-sequencing data. Electrophoresis. 31(21): 3537-44. PubMed Abstract 

Chen, X. et. al. (June 2010). Similar DNA Methylation and Histone H3 Lysine 9 Dimethylation Patterns in Tripronuclear and Corrected Bipronuclear Human Zygotes. J Reprod Dev. 56(3): 324-9. PubMed Abstract

Chen, C. et. al. (June 2009). Aberrant DNA methylation in thymic epithelial tumors. Cancer Investigation. 27(5): 582-91. PubMed Abstract

Roth, T. L. et. al. (May 2009). Lasting Epigenetic Influence of Early-Life Adversity on the BDNF Gene. Biol Psychiatry. 65(9): 760-9. Full PDF Article

Tsuji, Y. et. al. (May 2009). The developmental potential of mouse somatic cell nuclear-transferred oocytes treated with trichostatin A and 5-aza-2'-deoxycytidine. Zygote. 17(2): 109-15. PubMed Abstract

Weng, Y. I. et. al. (2009). Methylated DNA immunoprecipitation and microarray-based analysis: detection of DNA methylation in breast cancer cell lines. Methods Mol Biol. 590: 165-76. PubMed Abstract

Yun, X. X. et. al. (2009). DNA methylation patterns of mouse tetraploid embryos. Hereditas (Beijing). 31(4):387-392. Full PDF Article

Lubin, F. D. et. al. (October 2008). Epigenetic Regulation of bdnf Gene Transcription in the Consolidation of Fear Memory. J Neurosci. 28(42): 10576-86. PubMed Abstract

Novikova, S. I. et. al. (April 2008). Maternal Cocaine Administration in Mice Alters DNA Methylation and Gene Expression in Hippocampal Neurons of Neonatal and Prepubertal Offspring. PLoS ONE. 3(4): e1919. Full PDF Article

Mandrioli, M. et. al. (November 2007). Analysis of heterochromatic epigenetic markers in the holocentric chromosomes of the aphid Acyrthosiphon pisum. Chromosome Research. 15(8): 1015-22. PubMed Abstract