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HP1-gamma Polyclonal Antibody

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Western blot analysis of extracts of various cell lines, using HP1-gamma Polyclonal Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human lung cancer using HP1-gamma Polyclonal Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric using HP1-gamma Polyclonal Antibody at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using HP1-gamma Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Application: IF, IHC, WB
Areas of Research: Chromatin Remodeling
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Knockout Validated: Yes
Purification: Affinity Purified
Reactivity: Human, Mouse
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-4017-05050 µl $253.00 
A-4017-100100 µl $486.00 
Availability: Usually Ships in 3-4 Days 
Product Overview

Background
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.

Description
HP1-gamma Polyclonal Antibody. Unconjugated. Raised in Rabbit

Purification
Affinity Purified

Specificity
Mouse, Human

Isotype
IgG

Uniprot ID
Q13185

Formulation
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage
Store at -20℃. Avoid freeze / thaw cycles

Alternative Names
CBX 3 antibody, Chromobox homolog 3 (HP1 gamma homolog, Drosophila) antibody, Chromobox protein homolog 3 antibody, GAMMA antibody, HECH antibody, Heterochromatin protein 1 homolog gamma antibody, Heterochromatin protein HP1 gamma antibody, HP1 gamma antibody, HP1Hs gamma antibody, Modifier 2 protein antibody, HECH, HP1-GAMMA, HP1Hs-gamma

Application
WB (1:500 - 1:2000), IHC (1:50 - 1:200), IF (1:50 - 1:200)

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

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