SDHB Recombinant Monoclonal Antibody [7D12]

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Overlay histogram showing 293 cells stained with SDHB rMAb (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10⁶ cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*10⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.

IHC image of SDHB rMAb diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6. 0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0. 05% DAB.

IHC image of SDHB rMAb diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6. 0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0. 05% DAB.

Western Blot
Positive WB detected in: 293 whole cell lysate, K562 whole cell lysate, NIH/3T3 whole cell lysate, PC-3 whole cell lysate, Rat heart tissue, Mouse heart tissue, Mouse liver tissue
All lanes: SDHB antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa

Application:	ELISA, FC, IHC, WB
Areas of Research:	Other Primary Antibodies
Clonality:	Monoclonal
Conjugate:	Unconjugated
Host:	HEK293F Cell, Mouse
Isotype:	Rabbit IgG
Purification:	Affinity Chromatography
Reactivity:	Human, Rat
Recombinant:	Yes
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A74357-050	50µl	$299.00
A74357-100	100µl	$399.00

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Product Overview
Datasheet & SDS
Product FAQ

Product Overview

Background
Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).

Description
SDHB Recombinant Monoclonal Antibody [7D12]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human, Mouse, Rat

Isotype
Rabbit IgG

Uniprot ID
P21912

Purification
Affinity Chromatography

Immunogen
A synthesized peptide derived from human SDHB

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (EC 1.3.5.1) (Iron-sulfur subunit of complex II) (Ip), SDHB, SDH SDH1

Application
ELISA, WB, IHC, FC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, FC:1:20-1:200

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

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SDHB Recombinant Monoclonal Antibody [7D12]