iNOS Polyclonal Antibody

Background
Nitric oxide (NO) is a diffusible free radical gas that acts as a biological messenger between cells. Due to its rapid reaction with different substances, it has an exceedingly short half-life of only a few seconds. NO is synthesized in many tissues and affects a variety of physiological functions. Production of NO is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Three known isoforms of these enzymes exist: nNOS, type I (neuronal); eNOS, type III (endothelial cell); and iNOS, type II (inducible). All of these isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Inducible nitric oxide synthase (iNOS), which produce large amounts of NO, is expressed in a broad range of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Inflammatory mediators such as cytokines and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents also display the same effect on iNOS activity.

Formulation
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Immunogen
Synthetic peptide corresponding to aa 1131-1144 of mouse iNOS

Specificity
Mouse, Rat, Bovine

Purification
Protein A-affinity purified

Storage
Store at -20°C. Avoid multiple freeze/thaw cycles.

Alternative Names
NOS2, NOS II, Nitric oxide synthase (inducible), Hepatocyte NOS

Application
IP, WB (1:1000, ECL)

Fig. 1. Western blot analysis: rat brain extract, probed with iNOS Polyclonal Antibody.