The EpiQuik™ Tri-Methyl-Histone H3-K9 ChIP Kit is a convenient package of tools that allows the experimenter to investigate interactions of histone H3-K9 tri-methylation and DNA in vivo efficiently. The entire procedure can be completed within 5 hours and produces far superior results than any competitor kits. The EpiQuik™ Tri-Methyl-Histone H3-K9 ChIP Kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot as well as DNA microarray.

WHY CHOOSE THE EPIQUIK™ TRI-METHYL-HISTONE H3-K9 ChIP KIT?

• The fastest procedure available, which can be finished within 5 hours.
• Strip microplate format makes the assay flexible: manual or high throughput.
• Columns for DNA purification are included: save time and reduce labor.
• Compatible with all DNA amplification-based approaches.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ Tri-Methyl-Histone H3-K9 ChIP Kit contains all reagents required for carrying out a successful chromatin immunoprecipitation for tri-methyl-histone H3-K9 from mammalian cells. Particularly, this kit includes a ChIP-grade tri-methyl-histone H3-K9 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared, and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured tri-methyl-histone H3-K9 protein-DNA complex, reversed and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.

SCHEMATIC PROCEDURE:

CP1 (wash buffer)
CP2 (antibody buffer)
CP3A (pre-lysis buffer)
CP3B (lysis buffer)
CP4 (CHIP dilution buffer)
CP5 (DNA release buffer)
CP6 (reverse buffer)
CP7 (binding buffer)
CP8 (elution buffer)
Protease inhibitor cocktail (100x)*
Proteinase K (10 mg/ml)*
Normal mouse IgG (0.5 mg/ml)*
Anti-tri-methyl-H3-K9 (1 mg/ml)*
8-well assay strips (with frame)
8-well strip caps
F-spin column
F-collection tube
User guide

* Spin the solution down to the bottom before use.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.