We recommend using a newer version of this product: Epigenase LSD1 Demethylase Activity/Inhibition Assay Kit (Fluorometric)

 

The EpiQuik™ Histone Demethylase LSD1 Activity/Inhibition Assay Kit is a convenient set of tools that allows the experimenter to measure activity/inhibition of LSD1. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase LSD1 activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.

WHY CHOOSE THE EPIQUIK™ HISTONE DEMETHYLASE LSD1 ACTIVITY/INHIBITION ASSAY KIT?

• Fast procedure, which can be finished within 3 hours.
• Innovative fluorescent assay without the need for radioactivity, extraction, and chromatography.
• Direct measurement of LSD1 activity and not interfered by thiol-containing chemicals such as DTT, GSH and 2-mercaptoethanol.
• Both cell/tissue extracts and purified LSD1 can be used, which allows for detection of inhibitory effects of LSD1 inhibitor in vivo and in vitro.
• Strip microplate format makes the assay flexible: manual or high throughput analysis.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ Histone Demethylase LSD1 Activity/Inhibition Assay Kit is designed for measuring cellular LSD1 activity/inhibition. In the assay with this kit, the unique di-methylated histone H3-K4 substrate is stably captured on the strip wells. Active LSD1 binds to and demethylates histone H3-K4 substrate. The remaining un-demethylated substrate can be recognized with a high affinity anti-methylated histone H3-K4 antibody. The ratio or amount of the un-demethylated histone, which is inversely proportional to LSD1 activity, can then be fluorometrically quantified.

SCHEMATIC PROCEDURE:

HG1 (10X Wash Buffer)
HG2 (LSD1 Assay Buffer)
HG3 (LSD1 Substrate)*
HG4 (LSD1 Standard, 20 µg/ml)*
HG5 (Capture Antibody, 1000 µg/ml)*
HG6 (Detection Antibody, 200 µg/ml)*
HG7 (Fluoro-Developer)
8-Well Assay Strip (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.