In mammals, 5-hydroxymethylcytosine (5-hmC), classified as a sixth DNA base, can be generated via oxidation of 5-methylcytosine (5mC). This oxidation reaction is mediated by the ten-eleven translocation (TET) family of 5-mC-hydroxylases and further oxidation of 5-hmC results in 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) through TET hydroxylases. 5-fC has now been classified as a seventh DNA base. Although its function is not entirely clear, evidence demonstrates that it exhibits replication-dependent dilution during mouse pre-implantation development and could potentially function in regulating overall pre-implantation development. Because 5-mC is oxidized into 5-formylcytosine, 5-fC could potentially act as a marker for active DNA demethylation.
EpiGentek’s ELISA-based Global 5-fC Quantification assay service allows for the precise quantification of total 5-formylcytosine levels in DNA.
We currently accept DNA, cells, and tissue samples for our Global 5-fC Quantification service. Proper sample preparation along with the appropriate quality control methods on your end will allow the greatest chance for assay success. Please follow all guidelines for sample requirements, packaging and labeling, and shipping which are outlined on the Submission Instructions page.
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