Targeted Bisulfite Sequencing (Methyl-Seq) Service

Targeted Bisulfite Sequencing is an NGS method used to evaluate site specific DNA methylation changes. Our next-generation sequencing platforms can deliver a great amount of useful DNA methylation information with publication-ready data parsed by our expert bioinformatics scientists. The methylation analysis at single base resolution of individual cytosine in DNA is facilitated by bisulfite treatment of DNA followed by PCR amplification of targeted region, library construction, and sequencing of the amplicon regions. Specific primers are designed for the region of interest and cytosine methylation changes are evaluated within that region. Each DNA methylation site of interest receives high-sequencing depth of coverage for accurate, quantitative and single-base resolution data output.

EpiGentek’s Targeted Methyl-Seq platform yields reliable information on the methylation states of individual cytosines within the targeted region by effectively and efficiently preparing converted DNA for use in next generation sequencing techniques and offers the following advantages:

  • Complete conversion (>99%) and maximum recovery of bisulfite-converted DNA
  • Minimal selection bias, allowing for a low amount of input material high yield of the constructed DNA library, and low error rates
  • Streamlined process for rapid turnaround time
Targeted Bisulfite-Seq
Service #S-1TMS
Bisulfite sequencing method to detect in-depth DNA methylation at a targeted region of interest.
Bisulfite treatment
Primer design for targeted region
PCR amplification
NGS library prep
Illumina-based NGS sequencing
Bioinformatics analysis

Example Data

Targeted Bisulfite-Seq (Methyl-Seq) Example Data & Figures
*These are examples of data that may be provided. Please consult your service representative regarding the exact data you will receive.

Sample Requirements

Epigenetic Services Sample Requirements
We currently accept DNA, cell, and tissue samples for our Targeted Bisulfite Sequencing Methyl-Seq service. Proper sample preparation along with the appropriate quality control methods on your end will allow the greatest chance for assay success. Please follow all guidelines for sample requirements, packaging and labeling, and shipping which are outlined on the Submission Instructions page.

Talk to a Scientist

Want more information on our Targeted Bisulfite Sequencing Methyl-Seq service or interested in customizing your research project? We’re here to help! Get in touch with one of our highly knowledgeable epigenetic services experts by calling toll-free at 1-877-374-4368 or emailing services @