BackgroundThis methylase recognizes the double-stranded sequence GAGCTC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the SacI endonuclease.
DescriptionsacIM Polyclonal Antibody, Biotin Conjugated. Biotin. Raised in: Rabbit.
FormulationLiquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.
SpecificityStreptomyces achromogenes
IsotypeIgG
Uniprot IDO31073
Purification>95%, Protein G purified
ImmunogenRecombinant Streptomyces achromogenes Modification methylase SacI protein (1-390AA)
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesCytosine-specific methyltransferase SacI, sacIM
ApplicationELISA; Recommended dilution: ELISA 1:500-1:1000