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RAD18 Polyclonal Antibody

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Western blot
All lanes: RAD18 Polyclonal Antibody at 2ug/ml+COLO205 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 56kDa
Observed band size: 56kDa
Immunohistochemistry of paraffin-embedded human testis using RAD18 Polyclonal Antibody at dilution 1: 100
Application: ELISA, IHC, WB
Areas of Research: DNA Damage & Repair
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A56282-02020 µgView
A56282-05050 µgView
A56282-100100 µgView
Product Overview

Background
E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on Lys-164. Has ssDNA binding activity.

Description
RAD18 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
Q9NS91

Purification
>95%, Protein G purified

Immunogen
Recombinant Human E3 ubiquitin-protein ligase RAD18 protein (1-495AA)

Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Postreplication repair protein RAD18, RING finger protein 73, RAD18, RNF73

Application
ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200

User Guide & MSDS

[User Guide]*
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