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POU5F1 Monoclonal Antibody [4F8]

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Application: ELISA, WB
Areas of Research: Transcription Factors
Clonality: Monoclonal
Conjugate: Unconjugated
Host: Mouse
Isotype: IgG2a Kappa
Purification: Affinity Purified
Reactivity: Human
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-2236-100100 µg $452.00  Discontinued
Availability: Discontinued 
Product Overview

Description
Mouse monoclonal antibody raised against a full length recombinant POU5F1, clone 4F8.

Size
100 µg

Purification
Protein A purified

Immunogen
POU5F1 (AAH20712, 81 a.a. ~ 164 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG2a Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Alternative Names
Octamer binding transcription factor 4 antibody, MGC22487 antibody, Oct 3 antibody, Oct 4 antibody, Octamer binding protein 3 antibody, Octamer binding protein 4 antibody, OTF 3 antibody, OTF 4 antibody, POU class 5 homeobox 1 antibody, POU domain antibody, POU domain class 5 transcription factor 1 antibody, POU domain transcription factor OCT4 antibody

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (35.24 KDa).

 

Note: This item was formerly catalog no. ABB-2278 (ABB2278)

Product Details

Applications

Western Blot


POU5F1 Monoclonal Antibody, clone 4F8. Western Blot analysis of POU5F1 expression in HeLa.


Western Blot analysis of POU5F1 expression in transfected 293T cell line by POU5F1 Monoclonal Antibody, clone 4F8.

Lane 1: POU5F1 transfected lysate (18.3 KDa).
Lane 2: Non-transfected lysate.

Immunoprecipitation Not Determined

ELISA

Applicable

Immunohistochemistry (IHC)

Not Determined

Immunofluorescence

Not Determined
User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

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