DescriptionMouse monoclonal antibody raised against a full length recombinant POU5F1, clone 1D2.
Size100 µg
PurificationProtein A purified
ImmunogenPOU5F1 (AAH20712, 81 a.a. ~ 164 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross ReactivityHuman
IsotypeIgG2b Kappa
FormulationIn 1x PBS, pH 7.2
StorageAliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.
Handling RecommendationsFor maximum recovery of the products, centrifuge the vial prior to opening the cap.
Alternative NamesOctamer binding transcription factor 4 antibody, MGC22487 antibody, Oct 3 antibody, Oct 4 antibody, Octamer binding protein 3 antibody, Octamer binding protein 4 antibody, OTF 3 antibody, OTF 4 antibody, POU class 5 homeobox 1 antibody, POU domain antibody, POU domain class 5 transcription factor 1 antibody, POU domain transcription factor OCT4 antibody
Applications & Suggested Dilutions*Western Blot: 0.5-1 µg/mlELISA: 0.25-1 µg/ml
*The end user is responsible for determining optimal working dilutions.
Quality ControlWestern Blot detection against Immunogen (35.24 KDa).
Note: This item was formerly catalog no. ABB-2277 (ABB2277)
Applications
Western Blot
POU5F1 monoclonal antibody, clone 1D2. Western Blot analysis of POU5F1 expression in HepG2.
Western Blot analysis of POU5F1 expression in transfected 293T cell line by POU5F1 Monoclonal Antibody, clone 1D2.Lane 1: POU5F1 transfected lysate (18.3 KDa).Lane 2: Non-transfected lysate.
ELISA
Immunohistochemistry (IHC)
Immunofluorescence