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Histone phosphorylation has been shown to be involved in a variety of processes such as transcription, DNA repair, apoptosis, and chromosome condensation. Phosphorylation of serine 10 and 28 in histone H3 correlates with gene activation in mammalian cells, and subsequently causes an increase in HAT activity, resulting in transcriptional activation in genes.
Histone Phosphorylation Assay
Histone phosphorylation of histone H3 at serine 10 and serine 28 is conserved through eukaryotes, and an increase in phosphorylation has been shown to correlate with gene activation and cell growth. Detection of changes in histone phosphorylation levels can provide useful information in better understanding the pathological processes of some diseases, and for protein kinase-targeted drug developme...

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