Phospho Histone H3 (Ser10) Monoclonal Antibody

Background 
Histone H3– along with H2A, H2B, and H4– is involved in the structure of chromatin in eukaryotic cells. Histone H3 can undergo several different types of epigenetic modifications that influence cellular processes. These modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occur on the N-terminal tail domains of histone H3, which results in remodeling of the nucleosome structure into an open conformation more accessible to transcription complexes. In most species, histone H3 is primarily phosphorylated at serine 10, serine 28, threonine 3, and theonine11.

Description
Mouse monoclonal antibody raised against a synthetic peptide corresponding to the phosphorylated histone H3 at Ser10, ChIP-grade

Concentration
1 mg/ml

Purification
Protein A purified

Specificity
Detects histone H3 only when phosphorylated at Ser10 in mouse, rat, and human. Broad species cross-reactivity predicted based on sequence homology

Isotype
IgG

Formulation 
PBS (pH 7.5), 150 mM NaCl, 30% glycerol

Storage
Store at -20°C. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance

Alternative Names
H3ser10P antibody

Application 
WB: 1:1000-1:2000, IF: 1:100-1:500, IHC: 1:100-1:500, ELISA: 1:1000-1:2000, IP: 2 μg/10⁶ cells