BackgroundAKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al. , 2003 [PubMed 12524439]).
DescriptionPhospho-AKT1S1/PRAS40-T246 Monoclonal Antibody. Unconjugated. Raised in: Rabbit.
FormulationBuffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
SpecificityHuman
IsotypeIgG
Uniprot IDQ96B36
PurificationAffinity Purified
ImmunogenSynthetic peptide. Please contact EpigenTek for more information.
StorageStore at -20°C. Avoid freeze / thaw cycles.
Alternative NamesLobe; PRAS40
ApplicationWB, ELISA; Recommended dilution: WB, 1:2000 - 1:9000; ELISA, Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.