DescriptionHuman MYC full-length ORF (ENSP00000259523, 1 a.a. - 439 a.a.) recombinant protein with GST-tag at N-terminal
Molecular Weight (kDa)75.2 kDa
Formulation50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer
SourceIn vitro wheat germ expression system
Storage Temp & ConditionsStore at -80°C. Aliquot to avoid repeated freezing and thawing
Purification NotesGlutathione Sepharose 4 Fast Flow
Application NotesEnzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant Protein); Antibody Production; Protein Array
Fig. 1. 12.5% SDS-PAGE stained with Coomassie Blue.
Fig. 2. Standard curve generated with recombinant human MYC protein by ELISA. MYC protein is bound to glutathione plate and detected with anti-MYC antibody followed by secondary antibody-HRP reaction.