For 400 reactions. In some cases, particularly when complex genomic DNA or chemical modified DNA is used as a PCR template, specific amplification cannot be generated although all experimental parameters are likely to be optimized. A template with GC-rich content or repeat codons is also difficult to amplify. Methylamp™ PCR Enhancer is specifically designed to enhance specificity and yield of PCR amplification including methylation specific-PCR amplification. The benefit in using Methylamp™ PCR Enhancer is that it may enable an amplification that had previously failed. Methylamp™ PCR Enhancer is also able to reduce problematic PCR artifacts by decreasing formation of secondary structures in the GC region.
Yamanaka KI et. al. (July 2018). Normal DNA methylation status in sperm from a somatic cell cloned bull and their fertilized embryos. Anim Sci J.