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Methyl-DNA Binding Protein ChIP

Methyl-CpG-binding domain protein 2 (MBD2) is a member of the MBD protein family and has been shown to catalyze demethylation by direclty removing methyl groups from 5-methylcytosine residues in DNA. Chromatin immunoprecipitation would be useful in the identification of silenced genes associated with MBD2, and can be combined with qualitative and quantitative PCR, MSP, DNA sequencing, DNA microarrays, and Southern blot to profile or map MBD2 binding patterns.
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