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KAT5 Polyclonal Antibody

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Western blot analysis of extracts of various cell lines, using KAT5 Polyclonal Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat AntiRabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using KAT5 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Application: IF, IHC, WB
Areas of Research: Histone Acetylation & Deacetylation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-3701-05050 µlView
A-3701-100100 µlView
Why EpiGentek AntibodiesApplication Guarantee for Antibodies
Product Overview

Background
KAT5 belongs to the MYST protein family that contains atypical zinc finger and histone acetyltransferase domains. Also, KAT5 contains a chromodomain. KAT5 is a catalytic subunit of the NuA4 histone acetyltransferase complex. The NuA4 HAT complex plays a role in transcriptional activation of select genes mainly by acetylation of nucleosomal histone H4 and H2A, which influence nucleosome-DNA interaction and promotes interaction of the modified histones with other proteins that could regulate transcription positively. This complex also involves in the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, DNA repair and apoptosis.

Description
KAT5 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Human, Rat

Isotype
IgG

Uniprot ID
Q92993

Purification
Affinity Purified

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human KAT5 (NP_006379.2).

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Alternative Names
ESA1, HTATIP, HTATIP1, PLIP, TIP, TIP60, cPLA2, KAT5

Application
WB, IHC, IF; Recommended dilution: WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Safety Data Sheet]
BisulPlus Loci 5mC & 5hmC Detection PCR kit
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