BackgroundThe protein encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Two alternatively spliced transcript variants encoding different isoforms have been observed.
DescriptionMouse monoclonal antibody raised against a partial recombinant ING3, clone 2C4.
Size100 µg
PurificationProtein A purified
ImmunogenING3 (NP_938008, 1 a.a. ~ 93 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross ReactivityHuman, Rat
IsotypeIgG2b Kappa
FormulationIn 1x PBS, pH 7.2
StorageAliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.
Handling RecommendationsFor maximum recovery of the products, centrifuge the vial prior to opening the cap.
Alternative Names1300013A07Rik antibody, Eaf 4 antibody, FLJ20089 antibody, Inhibitor of growth family member 3 antibody, MEAF4 antibody, p47 ING 3 antibody
Applications & Suggested Dilutions*Western Blot: 0.5-1 µg/mlELISA: 0.25-1 µg/mlImmunofluorescence: 5 µg/ml
*The end user is responsible for determining optimal working dilutions.
Quality ControlWestern Blot detection against Immunogen (36.23 KDa).
Note: This item was formerly catalog no. ABB-4054 (ABB4054)
Applications
Western Blot
ING3 Monoclonal Antibody [2C4]. Western Blot analysis of ING3 expression in PC-12.
ELISA
Immunohistochemistry (IHC)