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Histone H4K5ac (Acetyl H4K5) Monoclonal Antibody [RMC429B]

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Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate
All lanes: Histone H4K5ac (Acetyl H4K5) Monoclonal Antibody [RMC429B] at 1.05 ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 11 KDa
Observed band size: 11 KDa
Immunocytochemistry analysis ofHistone H4K5ac (Acetyl H4K5) Monoclonal Antibody [RMC429B] diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30min) with Histone H4K5ac (Acetyl H4K5) Monoclonal Antibody [RMC429B] at 1:65, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, ICC, IF, WB
Areas of Research: Histone Acetylation & Deacetylation
Clonality: Monoclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human
100% Guarantee: 6 months
Catalog No.SizePriceQty
A68408-05050 µlView
A68408-100100 µlView
Why EpiGentek AntibodiesApplication Guarantee for Antibodies
Product Overview

Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Description
Histone H4K5ac (Acetyl H4K5) Monoclonal Antibody, clone RMC429B. Unconjugated. Raised in: Rabbit.

Concentration
Inquire

Formulation
Liquid. Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
IgG

Uniprot ID
P62805

Purification
Affinity Purified

Immunogen
A synthesized peptide

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Histone H4, HIST1H4A, H4/A, H4FA, AND, HIST1H4B, H4/I, H4FI, AND, HIST1H4C, H4/G, H4FG, AND, HIST1H4D, H4/B, H4FB, AND, HIST1H4E, H4/J, H4FJ, AND, HIST1H4F, H4/C, H4FC, AND, HIST1H4H, H4/H, H4FH, AND, HIST1H4I, H4/M, H4FM, AND, HIST1H4J, H4/E, H4FE, AND, HIST1H4K, H4/D, H4FD, AND, HIST1H4L, H4/K, H4FK, AND, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, AND, HIST2H4B, H4/O, H4FO, HIST4H4, Acetyl-Histone H4 (K5)

Application
ELISA, WB, ICC, IF; Recommended dilution: WB: 1:500-1:2000, ICC: 1:50-1:500, IF: 1:30-1:200

VCU0006

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