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Histone H3R17 Monomethyl (H3R17me1) Polyclonal Antibody

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Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using Histone H3R17 Monomethyl Polyclonal Antibody.
Dot-blot analysis of all sorts of methylation peptides using Histone H3R17 Monomethyl Polyclonal Antibody.
Immunofluorescence analysis of 293T cell using Histone H3R17 Monomethyl Polyclonal Antibody. Blue: DAPI for nuclear staining.
Application: ChIP, ChIPseq, IF, IHC, IP, WB
Areas of Research: Histone Methylation & Demethylation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-3710-05050 µlView
A-3710-100100 µlView
Product Overview

Background
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase.

Description
Histone H3R17 Monomethyl (H3R17me1) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Specificity
Human, Mouse, Rat, Broad Range

Isotype
IgG

Uniprot ID
P68431

Purification
Affinity Purified

Immunogen
Synthetic Peptide of Human MonoMethyl-Histone H3-R17

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Alternative Names
H3R17me1, HIST1H3J, H3/j , H3FJ , Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h , Histone H3/I, Histone H3/j , Histone H3/k, Histone H3/l, H3 Arginine 17 me1

Application
WB, IHC, IF, IP, ChIP, ChIPseq

User Guide & MSDS

[User Guide]*
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