BackgroundThe primary building block of chromatin is the nucleosome which is made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4). Histones play a key role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated through an intricate series of post-translational modifications of histones, known as the histone code, and nucleosome remodeling. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. There are numerous well-characterized residues for phosphorylation within the N-terminal tail of histone H3; these include Thr-3, Ser-10, Thr-11, and Ser-28. Phosphorylation of histone H3 on Ser-10 in the N-terminal tail is considered essential during mitosis and meiosis for cell cycle progression and chromosome condensation.
DescriptionRabbit polyclonal antibody to Histone H3 (pSer10)
FormulationLiquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
ImmunogenSynthetic peptide corresponding to human Histone H3 phosphorylated at Ser10.
SpecificityHuman, Mouse, Rat, Chicken, Drosophila, Monkey, Pig, Rabbit
PurificationProtein A-affinity purified
StorageStore at -20°C. Avoid multiple freeze/thaw cycles.
ApplicationWB
Fig. 1. Western blot analysis of MW marker (1), untreated Jurkat (2), and calyculin A-treated Jurkat (3) cell lysates probed with Histone H3 (pSer10) Polyclonal Antibody.