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HDAC3 Polyclonal Antibody

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Western blot
All lanes: HDAC3 Polyclonal Antibody at 2ug/ml+Hela cells
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 49, 50 kDa
Observed band size: 49 kDa
Western Blot
Positive WB detected in: mouse heart
All lanes: HDAC3 Polyclonal Antibody at 2.5ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49, 50 kDa
Observed band size: 49 kDa
Immunohistochemistry of paraffin-embedded human breast cancer using HDAC3 Polyclonal Antibody at dilution 1:100
Chromatin Immunoprecipitation Hela (1.2*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 ug HDAC3 Polyclonal Antibody or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the P21 promoter.
Immunofluorescent analysis of Hela cells using HDAC3 Polyclonal Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Application: ChIP, ELISA, IF, IHC, WB
Areas of Research: Histone Acetylation & Deacetylation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human, Mouse
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A68305-02020 µgView
A68305-05050 µgView
A68305-100100 µgView
Product Overview

Background
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.

Description
HDAC3 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.

Specificity
Human, Mouse

Isotype
IgG

Uniprot ID
O15379

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Histone deacetylase 3 protein (1-428AA)

Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
HD3, RPD3-2, SMAP45, HDAC3

Application
ELISA, WB, IHC, IF, ChIP; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200

User Guide & MSDS

[User Guide]*
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