BackgroundHistone deacetylases (HDACs) play a critical role in transcriptional repression of gene expression in eukaryotic cells through catalyzing the hydrolytic removal of acetyl groups from histone lysine residues. Three distinct families of HDACs have been described, comprising a group of at least 20 proteins in humans. HDAC3 is a class I histone deacetylase.
DescriptionMouse monoclonal antibody raised against a partial recombinant HDAC3, clone 3E11.
Size 100 µg
PurificationProtein A purified
ImmunogenHDAC3 (NP_003874, 319 a.a. ~ 429 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross ReactivityHuman, Mouse, Rat
IsotypeIgG2a Kappa
FormulationIn 1x PBS, pH 7.2
StorageAliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.
Handling RecommendationsFor maximum recovery of the products, centrifuge the vial prior to opening the cap.
Alternative NamesHD 3 antibody, Histone deacetylase 3 (HD3) (RPD3-2) antibody, RPD 3 antibody, RPD3 2 antibody, SMAP 45 antibody
Applications & Suggested Dilutions*Western Blot: 0.5-1 µg/mlELISA: 0.25-1 µg/ml*The end user is responsible for determining optimal working dilutions.
Quality ControlWestern Blot detection against Immunogen (38.21 KDa).
Note: This item was formerly catalog no. ABB-4003 (ABB4003)
Applications
Western Blot
HDAC3 Monoclonal Antibody [3E11]. Western Blot analysis of HDAC3 expression in PC-12.
HDAC3 Monoclonal Antibody [3E11]. Western Blot analysis of HDAC3 expression in Hela NE.
HDAC3 Monoclonal Antibody [3E11]. Western Blot analysis of HDAC3 expression in Raw 264.7.
HDAC3 Monoclonal Antibody [3E11]. Western Blot analysis of HDAC3 expression in NIH/3T3.
ELISA
Immunohistochemistry (IHC)