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HDAC1 Polyclonal Antibody

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Western blot
All lanes: HDAC1 Polyclonal Antibody at 4ug/ml
Lane 1: mouse gonad tissue
Lane 2: 293T whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: jurkat whole cell lysate
Lane 5: HT29 whole cell lysate
Lane 6: A549 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 55kDa
Observed band size: 55kDa
Western blot
All lanes: HDAC1 Polyclonal Antibody at 3ug/ml
Lane 1: MCF7 whole cell lysate
Lane 2: Zebrafish lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 55 kDa
Observed band size: 55, 160 kDa
Immunohistochemistry of paraffin-embedded human testis at dilution 1:100
Immunohistochemistry of paraffin-embedded human colon cancer at dilution 1:100
Chromatin Immunoprecipitation MCF7(1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-HDAC1 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the P21 promoter.
Immunohistochemistry of paraffin-embedded human thyroid tissue at dilution of 1:100
Immunohistochemistry of paraffin-embedded human melanoma cancer at dilution of 1:100
Application: ELISA, IF, IHC
Areas of Research: Histone Acetylation & Deacetylation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A68304-02020 µgView
A68304-05050 µgView
A68304-100100 µgView
Product Overview

Background
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation.

Description
HDAC1 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
Q13547

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Histone deacetylase 1 protein (1-482AA)

Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Histone deacetylase 1, HD1, HDAC1, RPD3L1

Application
ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200

User Guide & MSDS

[User Guide]*
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