BackgroundHistone deacetylases (HDACs) play a critical role in transcriptional repression of gene expression in eukaryotic cells through catalyzing the hydrolytic removal of acetyl groups from histone lysine residues. Three distinct families of HDACs have been described, comprising a group of at least 20 proteins in humans. HDAC1 is a class I histone deacetylase.
DescriptionMouse monoclonal antibody raised against a full length recombinant HDAC1, clone 3E1.
Size100 µg
PurificationProtein A purified
ImmunogenHDAC1 (AAH00301, 1 a.a. ~ 483 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross ReactivityHuman, Mouse, Rat
IsotypeIgG1 Kappa
FormulationIn 1x PBS, pH 7.2
StorageAliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.
Handling RecommendationsFor maximum recovery of the products, centrifuge the vial prior to opening the cap.
Alternative NamesDKFZp686H12203 antibody, GON 10 antibody, HD 1 antibody, Histone deacetylase 1 antibody, Reduced potassium dependency yeast homolog like 1 antibody, RPD3 antibody, RPD3L1 antibody
Applications & Suggested Dilutions*Western Blot: 0.5-1 μg/mlELISA: 0.25-1 µg/mlImmunohistochemistry (IHC): 2.5 µg/mlImmunofluorescence: 5 μg/ml
*The end user is responsible for determining optimal working dilutions.
Quality ControlWestern Blot detection against Immunogen (79.13 KDa).
Note: This item was formerly catalog no. ABB-4001 (ABB4001)
Applications
Western Blot
HDAC1 Monoclonal Antibody, clone 3E1. Western Blot analysis of HDAC1 expression in PC-12.
HDAC1 Monoclonal Antibody, clone 3E1. Western Blot analysis of HDAC1 expression in HeLa NE.
HDAC1 Monoclonal Antibody, clone 3E1. Western Blot analysis of HDAC1 expression in Raw 264.7.
HDAC1 Monoclonal Antibody, clone 3E1. Western Blot analysis of HDAC1 expression in NIH/3T3.
ELISA
Immunohistochemistry (IHC)