Description Goat anti-rat IgG recognizes rat IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Biotin.
Antibody Type Polyclonal Antibody
Purification Liquid; This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.
Immunogen Rat IgG whole molecule
Isotype IgG
Formulation In 10 mM sodium phosphate, 75 mM NaCl, 50% (v/v) glycerol, pH 7.2.
Specificity Rat
Storage Store at 4°C. For long-term storage, store at -20°C. Aliquot to avoid repeated freezing and thawing.
Handling Recommendations The optimal working dilution should be determined by the end user. For maximum recovery of the products, centrifuge the vial prior to opening the cap.
Applications & Suggested Dilutions Western Blot: 1:1000-1: 10000; Immunohistochemistry: 1:100-1:500; Immunofluorescence: 1:100-1:500; ELISA: 1:2000-1:20000.