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EYA1 Polyclonal Antibody

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Immunofluorescence staining of Hela cells with EYA1 Polyclonal Antibody at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, IF, IHC
Areas of Research: Cell Development & Differentiation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A69151-02020 µgView
A69151-05050 µgView
A69151-100100 µgView
Why EpiGentek AntibodiesApplication Guarantee for Antibodies
Product Overview

Background
in this it is functionally redundant with EYA2.

Description
EYA1 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
Q99502

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Eyes absent homolog 1 protein (168-321AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Eyes absent homolog 1, EYA1

Application
ELISA, IHC, IF; Recommended dilution: IHC:1:500-1:1000, IF:1:50-1:200

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