DescriptionEpiQuik Pfu DNA Polymerase has been purified from the Recombinant E. coli strain with cloned gene encoding Pyrococcus furiosus DNA polymerase. In addition to 5´→3´ DNA polymerase activity, EpiQuik Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proof-reading) activity. EpiQuik Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, it is up to ten fold more accurate than normal Taq DNA polymerase. Consequently, EpiQuik Pfu DNA Polymerase is useful for polymerization reactions requiring high-fidelity synthesis.
Concentration5 units/µl (one unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C).
SourceRecombinant E. coli strain with cloned gene encoding Pyrococcus furiosus DNA polymerase.
Composition
Applications
Storage Conditions Store at -20°C. Guaranteed stable for 12 months when properly stored.