We recommend using a newer version of this product: Epigenase LSD1 Demethylase Activity/Inhibition Assay Kit (Fluorometric)
The EpiQuik™ Histone Demethylase LSD1 Activity/Inhibition Assay Kit is a convenient set of tools that allows the experimenter to measure activity/inhibition of LSD1. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase LSD1 activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.
WHY CHOOSE THE EPIQUIK™ HISTONE DEMETHYLASE LSD1 ACTIVITY/INHIBITION ASSAY KIT,
Fig. 1. LSD1 activity measurement with the EpiQuik™ Histone Demethylase LSD1 Activity/Inhibition Assay Kit . Recombinant human LSD1 enzyme was added at different concentrations. Original RFU data. RFU is inversely proportional to LSD1 enzyme activity.
Fig. 2. RFU data after conversion from Fig.1.
Fig. 3. LSD1 activity measured from NIH 3T3 cell extracts.
The EpiQuik™ Histone Demethylase LSD1 Activity/Inhibition Assay Kit is designed for measuring cellular LSD1 activity/inhibition. In the assay with this kit, the unique di-methylated histone H3-K4 substrate is stably captured on the strip wells. Active LSD1 binds to and demethylates histone H3-K4 substrate. The remaining un-demethylated substrate can be recognized with a high affinity anti-methylated histone H3-K4 antibody. The ratio or amount of the un-demethylated histone, which is inversely proportional to LSD1 activity, can then be fluorometrically quantified.