The EpiQuik™ Histone Demethylase LSD1 Activity/Inhibition Assay Kit is designed for measuring cellular LSD1 activity/inhibition. In the assay with this kit, the unique di-methylated histone H3-K4 substrate is stably captured on the strip wells. Active LSD1 binds to and demethylates histone H3-K4 substrate. The remaining un-demethylated substrate can be recognized with a high affinity anti-methylated histone H3-K4 antibody. The ratio or amount of the un-demethylated histone, which is inversely proportional to LSD1 activity, can then be fluorometrically quantified.
HG1 (10X Wash Buffer)
HG2 (LSD1 Assay Buffer)
HG3 (LSD1 Substrate)*
HG4 (LSD1 Standard, 20 µg/ml)*
HG5 (Capture Antibody, 1000 µg/ml)*
HG6 (Detection Antibody, 200 µg/ml)*
8-Well Assay Strip (with Frame)
* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.