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EpiQuik Histone Demethylase LSD1 Activity/Inhibition Assay Kit

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LSD1 activity measurement with the EpiQuik™ Histone Demethylase LSD1 Activity/Inhibition Assay Kit . Recombinant human LSD1 enzyme was added at different concentrations. Original RFU data. RFU is inversely proportional to LSD1 enzyme activity.
LSD1 activity measured from NIH 3T3 cell extracts.
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: Histone Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3076-4848 assays $266.00 
P-3076-9696 assays $452.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

We recommend using a newer version of this product: Epigenase LSD1 Demethylase Activity/Inhibition Assay Kit (Fluorometric)

 

The EpiQuik™ Histone Demethylase LSD1 Activity/Inhibition Assay Kit is a convenient set of tools that allows the experimenter to measure activity/inhibition of LSD1. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase LSD1 activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria. 

WHY CHOOSE THE EPIQUIK™ HISTONE DEMETHYLASE LSD1 ACTIVITY/INHIBITION ASSAY KIT,

  • Fast procedure, which can be finished within 3 hours.
  • Innovative fluorescent assay without the need for radioactivity, extraction, and chromatography.
  • Direct measurement of LSD1 activity and not interfered by thiol-containing chemicals such as DTT, GSH and 2-mercaptoethanol.
  • Both cell/tissue extracts and purified LSD1 can be used, which allows for detection of inhibitory effects of LSD1 inhibitor in vivo and in vitro.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Simple, reliable, and consistent assay conditions.


Fig. 1. LSD1 activity measurement with the EpiQuik™ Histone Demethylase LSD1 Activity/Inhibition Assay Kit . Recombinant human LSD1 enzyme was added at different concentrations. Original RFU data. RFU is inversely proportional to LSD1 enzyme activity.

Fig. 2. RFU data after conversion from Fig.1.

Fig. 3. LSD1 activity measured from NIH 3T3 cell extracts.

Product Details

The EpiQuik™ Histone Demethylase LSD1 Activity/Inhibition Assay Kit is designed for measuring cellular LSD1 activity/inhibition. In the assay with this kit, the unique di-methylated histone H3-K4 substrate is stably captured on the strip wells. Active LSD1 binds to and demethylates histone H3-K4 substrate. The remaining un-demethylated substrate can be recognized with a high affinity anti-methylated histone H3-K4 antibody. The ratio or amount of the un-demethylated histone, which is inversely proportional to LSD1 activity, can then be fluorometrically quantified.

SCHEMATIC PROCEDURE:  
 
Product Components

HG1 (10X Wash Buffer)
HG2 (LSD1 Assay Buffer)
HG3 (LSD1 Substrate)*
HG4 (LSD1 Standard, 20 µg/ml)*
HG5 (Capture Antibody, 1000 µg/ml)*
HG6 (Detection Antibody, 200 µg/ml)*
HG7 (Fluoro-Developer)
8-Well Assay Strip (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

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