Epigentek Home
 Find Your Distributor      
 

EpiQuik Global Tri-Methyl Histone H4K20 Quantification Kit (Colorimetric)

Share:

For ELISA-like measurement of total H4K20me3 amounts

Citations (1) | Write a Review
Suggested Workflow
Histone Protein Extraction
 
 
Histone H4 Methylation Quantification
 
Schematic procedure for using the EpiQuik Global Tri-Methyl Histone H4K20 Quantification Kit (Colorimetric).
Input Type: Histone Extracts
Research Area: Histone Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3068-4848 assaysView
P-3068-9696 assaysView
Product Overview

The EpiQuik™ Global Tri-Methyl Histone H4-K20 Quantification Kit (Colorimetric) is a complete set of essential components, which enables the experimenter to measure tri-methylation of histone H4-K20. The EpiQuik Global Tri-Methyl Histone H4-K20 Quantification Kit (Colorimetric) is suitable for using a variety of mammalian cells (human, mouse, etc.), including fresh and frozen tissues, and cultured adherent and suspension cells. The kit has the following advantages:

  • Quick and efficient procedure, which can be finished within 2.5 hours.
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography.
  • Specifically captures tri-methylated H4-K20 with the detection limit as low as 5 ng/well and detection range from 20 ng to 2 µg/well of histone extracts.
  • The control is conveniently included for the quantification of tri-methylated H4-K20.
  • Strip microplate format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.

Background Information
Epigenetic activation or inactivation of genes plays a critical role in many important human diseases, especially in cancer. A major mechanism for epigenetic inactivation of the genes is methylation of CpG islands in genome DNA caused by DNA methyltransferases. Histone methyltransferases (HMTs) control or regulate DNA methylation through chromatin-dependent transcriptional repression or activation. HMTs transfer 1-3 methyl groups from S-adenosyl-Lmethionine to the lysine and arginine residues of histone proteins. PR-SET7, SET9, SUV4.20h, and ASH1are histone methyltransferases that catalyze methylation of histone H4 at lysine 20 (H4-K20) in mammalian cells. Tri-methylation of H4-K20 acts as a passive feature or structure determinant for chromatin degradation and release, as well as being an epigenetic marker of early apoptosis. Tri-methylation of H4-K20 is also considered as a common hallmark of human cancer. The global H4-K20 tri-methylation can be changed by inhibition or activation of HMTs. Therefore, quantitative detection of global tri-methyl histone H4-K20 would provide useful information for better understanding epigenetic regulation of tumorigenesis and apoptosis, as well as for developing HMT-targeted drugs. The EpiQuik™ Global Tri-Methyl Histone H4-K20 Quantification Kit (Colorimetric) provides a tool for measuring tri-methylation of histone H4-K20.

Principle & Procedure
This kit is designed for measuring global histone H4-K20 tri-methylation. In an assay with this kit, the tri-methylated histone H4 at lysine 20 is captured to strip wells coated with an anti-trimethyl H4-K20 antibody. The captured tri-methylated histone H4-K20 can then be detected with a detection antibody, followed by a color development reagent. The ratio of tri-methylated H4-K20 is proportional to the intensity of absorbance. The absolute amount of tri-methylated H4-K20 can be quantitated by comparing to the standard control.

Starting Materials
Input material should be purified histone extracts. In general, the input amount should be from 50 ng to 200 ng per well of histone extracts. 

 

Product Components

C1 (10X Wash Buffer)
C2 (Antibody Buffer)
C3 (Detecting Antibody, 1 mg/ml)*
C4 (Color Developer)
C5 (Stop Solution)
Standard Control (100 µg/ml)*
Signal Report Solution*
Signal Enhancer*
8-Well Sample Strips (with Frame)
8-Well Standard Control Strips
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
[Safety Data Sheet]
Product Citations

Gezer U et. al. (December 2015). Histone Methylation Marks on Circulating Nucleosomes as Novel Blood-Based Biomarker in Colorectal Cancer Int J Mol Sci. 16(12):29654-29662.

DNMT ELISA Easy Kit
Related Products

Researchers Also Purchased
EpiQuik Total Histone Extraction Kit
EpiQuik Total Histone Extraction Kit
EpiQuik Total Histone H4 Quantification Kit (Colorimetric)
EpiQuik Total Histone H4 Quantification Kit (Colorimetric)
EpiQuik Global Tri-Methyl Histone H3K9 Quantification Kit (Colorimetric)
EpiQuik Global Tri-Methyl Histone H3K9 Quantification Kit (Colorimetric)
MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)
MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)
Epigentek is an epigenetics company that specializes in epigenetic kits, antibodies, reagents, and services for epigenetic research in DNA methylation, histone modification, and chromatin studies. Terms & Conditions | Privacy Policy | Site Map
Copyright © 2019 Epigentek Group Inc. All rights reserved.

Intro to Epigenetics