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DNA Methyltransferase & Demethylase Assays

DNA methyltransferases, or DNMTs, catalyze DNA methylation by adding methyl groups to the 5-carbon position of the cytosine ring, resulting in 5-methylcytosine. The various types of DNMTs are responsible for the maintenance and establishment of DNA methylation patterns. The ten-eleven translocation, or TET, family of 5-mC hydroxylase enzymes including TET1 are responsible for oxidizing 5-methylcytosine into 5-hydroxymethylcytosine. Measuring DNMT and TET amounts, activity, and inhibition levels will allow us to better understand the relationships between cytosine, 5-methylcytosine, and 5-hydroxymethylcytosine as part of the DNA methylation and DNA demethylation cycle.

 

EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric)Cat #P-3009

Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Colorimetric) Cat #P-3086

Epigenase Thymine DNA Glycosylase (TDG) Activity/Inhibition Assay Kit (Colorimetric) Cat #P-3094

EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra KitCat #P-3008

  More DetailsMore DetailsMore DetailsMore Details
PurposeColorimetrically measure total DNMT activity/inhibitionColorimetrically measure total TET activity/inhibitionColorimetrically measure total TDG activity/inhibitionColorimetrically measure total DNA demethylase activity/inhibition
Starting MaterialNuclear extract or purified enzymeNuclear extract or purified enzymeNuclear extract or purified enzymeNuclear extract
Input Range0.5-20 µg of nuclear extract; or 20-200 ng of purified enzyme2-20 µg of nuclear extract; or 20-1000 ng of purified enzyme2-10 µg of nuclear extract; or 20-1000 ng of purified enzyme2-20 µg of nuclear extract
Total Time4 hours5 hours5 hours4 hours
Detection Threshold0.5 ng of purified DNMT20 ng of purified TET20 ng of purified TDG1 µg of nuclear extract
Positive Control Included
FormatELISA-like Microplate-basedELISA-like Microplate-basedELISA-like Microplate-basedELISA-like Microplate-based
Result Pages:  1  Displaying 1 to 15 (of 15 products)
Image Description Cat. No. Size Price
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit

Citations (126)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3001-148 assaysView
P-3001-296 assaysView
Research Kits
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit (Fluorometric)
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit (Fluorometric)

Input Type: DNA
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3004-4848 assaysView
P-3004-9696 assaysView
Research Kits
EpiQuik DNMT1 Activity/Inhibitor Screening Assay Core Kit
EpiQuik DNMT1 Activity/Inhibitor Screening Assay Core Kit

Citations (1)
Input Type: Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3006A-4848 assaysView
P-3006A-9696 assaysView
Research Kits
EpiQuik DNMT3B Activity/Inhibitor Screening Assay Core Kit
EpiQuik DNMT3B Activity/Inhibitor Screening Assay Core Kit

Input Type: Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3007A-4848 assaysView
P-3007A-9696 assaysView
Research Kits
EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra Kit
EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra Kit

Citations (2)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3008-4848 assaysView
P-3008-9696 assaysView
Research Kits
EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric)
EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric)

Citations (54) | (2)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3009-4848 assaysView
P-3009-9696 assaysView
Research Kits
EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Fluorometric)
EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Fluorometric)

Citations (7)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3010-4848 assaysView
P-3010-9696 assaysView
Research Kits
EpiQuik Dnmt1 Assay Kit
EpiQuik Dnmt1 Assay Kit

Citations (22)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-3011-248 assaysView
P-3011-396 assaysView
Research Kits
EpiQuik Dnmt3A Assay Kit
EpiQuik Dnmt3A Assay Kit

Citations (6)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-3012-248 assaysView
P-3012-396 assaysView
Research Kits
EpiQuik Dnmt3B Assay Kit
EpiQuik Dnmt3B Assay Kit

Citations (8)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-3013-248 assaysView
P-3013-396 assaysView
Research Kits
EpiQuik DNA Demethylase Activity/Inhibition Assay Kit
EpiQuik DNA Demethylase Activity/Inhibition Assay Kit

Citations (1)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3019-4848 assaysView
P-3019-9696 assaysView
Research Kits
Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Colorimetric)
Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Colorimetric)

Citations (14)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3086-4848 assaysView
P-3086-9696 assaysView
Research Kits
Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Fluorometric)
Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Fluorometric)

Citations (3)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3087-4848 assaysView
P-3087-9696 assaysView
Research Kits
Epigenase Thymine DNA Glycosylase (TDG) Activity/Inhibition Assay Kit (Colorimetric)
Epigenase Thymine DNA Glycosylase (TDG) Activity/Inhibition Assay Kit (Colorimetric)

Citations (2)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3094-4848 AssaysView
P-3094-9696 AssaysView
Research Kits
Epigenase m6A Demethylase Activity/Inhibition Assay Kit (Colorimetric)
Epigenase m6A Demethylase Activity/Inhibition Assay Kit (Colorimetric) new product

Input Type: Nuclear Extracts or Purified Enzymes
Research Area: RNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-9013-4848 assaysView
P-9013-9696 assaysView
Research Kits
Result Pages:  1  Displaying 1 to 15 (of 15 products)
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