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CLCNKB Polyclonal Antibody

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Western Blot
Positive WB detected in: Hela whole cell lysate, Rat kidney tissue
All lanes: CLCNKB antibody at 3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 76, 57 KDa
Observed band size: 76 KDa
IHC image of CLCNKB Polyclonal Antibody diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CLCNKB Polyclonal Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, IF, IHC, WB
Areas of Research: Signal Transduction
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human, Rat
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A69439-02020 µgView
A69439-05050 µgView
A69439-100100 µgView
Product Overview

Background
membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms.

Description
CLCNKB Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human, Rat

Isotype
IgG

Uniprot ID
P51801

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Chloride channel protein ClC-Kb protein (538-687AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Chloride channel protein ClC-Kb, Chloride channel Kb, ClC-K2, CLCNKB

Application
ELISA, WB, IHC, IF; Recommended dilution: WB: 1:500-1:5000, IHC: 1:200-1:500, IF: 1:50-1:200

User Guide & MSDS

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