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ATG4A Polyclonal Antibody

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Western blot
All lanes: ATG4A Polyclonal Antibody at 2ug/ml+mouse brain tissue
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 45kDa
Observed band size: 45kDa
Application: ELISA, WB
Areas of Research: Apoptosis & Cell Death
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Protein G Purified
Reactivity: Human, Mouse
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A51133-02020 µgView
A51133-05050 µgView
A51133-100100 µgView
Product Overview

Background
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Description
ATG4A Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.

Specificity
Human, Mouse

Isotype
IgG

Uniprot ID
Q8WYN0

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Cysteine protease ATG4A protein (1-214AA)

Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
AUT-like 2 cysteine endopeptidase, Autophagin-2, Autophagy-related cysteine endopeptidase 2, Autophagy-related protein 4 homolog A, hAPG4A, ATG4A, APG4A, AUTL2

Application
ELISA, WB; Recommended dilution: WB:1:200-1000

User Guide & MSDS

[User Guide]*
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