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Histone H3K79me3 (H3K79 Trimethyl) Polyclonal Antibody

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Citations (3) | Write a Review
Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using Histone H3K79me3 Polyclonal Antibody.
Dot-blot analysis of all sorts of methylation peptides using Histone H3K79me3 Polyclonal Antibody.
Immunohistochemistry of paraffin-embedded rat brain tissue using Histone H3K79me3 Polyclonal Antibody at dilution of 1:200 (x400 lens).
Immunohistochemistry of paraffin-embedded rat kidney tissue using Histone H3K79me3 Polyclonal Antibody at dilution of 1:200 (x400 lens).
Immunohistochemistry of paraffin-embedded human lung cancer tissue using Histone H3K79me3 Polyclonal Antibody at dilution of 1:200 (x400 lens).
Immunohistochemistry of paraffin-embedded human rectal cancer tissue using Histone H3K79me3 Polyclonal Antibody at dilution of 1:200 (x400 lens).
Immunofluorescence analysis of 293T cell using Histone H3K79me3 Polyclonal Antibody. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis extracts of 293 cell line, using Histone H3K79me3 Polyclonal Antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Application: ChIP, ChIPseq, IF, IHC, IP, WB
Areas of Research: Histone Methylation & Demethylation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-4045-02525 µlView
A-4045-05050 µlView
Product Overview

Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Description
Histone H3K79me3 (H3K79 Trimethyl) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Broad Range, Mouse, Rat, Human

Isotype
IgG

Uniprot ID
Q16695

Purification
Affinity Purified

Immunogen
Synthetic Peptide of Human TriMethyl-Histone H3-K79

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Application
WB, IHC, IF, IP, ChIP, ChIP-seq; Recommended dilution: WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200, IP 1:50 - 1:200, ChIP 1:20 - 1:100, ChIP-seq 1:20 - 1:100

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Safety Data Sheet]
DNMT ELISA Easy Kit
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