Histone H3K18ac (Acetyl H3K18) Polyclonal Antibody

Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21. 3.

Description
Histone H3K18ac (Acetyl H3K18) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3

Specificity
Broad Range, Mouse, Rat, Human

Isotype
IgG

Purification
Affinity Purified

Immunogen
A synthetic acetylated peptide around K9 of human Histone H3 (NP_003520.1).

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Alternative Names
H3K18ac antibody; H3K18a antibody; H3t; H3.4; H3/g; H3FT

Application
WB, IHC, IF/ICC, IP, ChIP, ChIPseq; Recommended dilution: WB 1:100 - 1:500, IHC 1:200 - 1:500, IF/ICC 1:500 - 1:1000, IP 1:200 - 1:500, ChIP 1:50 - 1:100, ChIP-seq 1:50 - 1:100