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AGO2 Polyclonal Antibody

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Western blot
All Lanes: AGO2 Polyclonal Antibody at 2ug/ml
Lane 1: A549 whole cell lysate
Lane 2: Jurkats whole cell lysate
Lane 3: MCF-7 whole cell lysate
Lane 4: HepG2 whole cell lysate
Lane 5: Raw264.7 whole cell lysate
Lane 6: K562 whole cell lysate
Lane 7: Mouse liver tissue
Lane 8: Mouse kidney tissue
Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 97 kDa
Observed band size: 90 kDa
Immunohistochemistry of paraffin-embedded human breast cancer using AGO2 Polyclonal Antibody at dilution 1:100
Immunohistochemistry of paraffin-embedded human breast cancer using AGO2 Polyclonal Antibody at dilution 1:100
Immunohistochemistry of paraffin-embedded human gastric cancer using AGO2 Polyclonal Antibody at dilution 1:100
Chromatin Immunoprecipitation Hela(1.1*106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-AgO2 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the SLC1A5 promoter.
Application: ELISA, IHC
Areas of Research: RNA Processing and Modification
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A68299-02020 µgView
A68299-05050 µgView
A68299-100100 µgView
Product Overview

Background
Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The minimal RISC appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.

Description
AGO2 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
Q9UKV8

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Protein argonaute-2 protein (517-818AA)

Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C 2, PAZ Piwi domain protein, Protein slicer, EIF2C2, AGO2

Application
ELISA, IHC; Recommended dilution: IHC:1:100-1:1200

User Guide & MSDS

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