DescriptionSheep polyclonal antibody to 14-3-3 (pSer185)
Immunogen14-3-3 phosphorylated at Ser185.
SpecificityHuman, Mouse, Rat, Cow, Sheep
PurificationPhospho-specific antibody
StorageStore at -80°C. Avoid multiple freeze/thaw cycles.
Alternative NamesKCIP-1, Protein kinase C inhibitor protein 1, 14-3-3β/ζ, Protein 1054
ApplicationWB (1:300-500)
Fig. 1. Western blot analysis performed with 14-3-3 (pSer185) Polyclonal Antibody (1:500), secondary anti-sheep antibody (1:2’000), blotted against 2μg each of: 1. Recombinant 14-3-3β expressed in E.coli 2. Recombinant 14-3-3ζ expressed in E.coli 3. Sheep brain homogenate, which contains ~50% of β and ζ isoforms that are phosphorylated on Ser185.