5-hmC Sequencing Service

Until the recent discovery of 5-hydroxymethylcytosine (5-hmC) — a hydroxylated and methylated form of cytosine — in mammals, it was believed that 5-methylcytosine (5-mC) was the only DNA base modification. Evidence shows that 5-hmC is predominately located within gene promoter regions and is associated with transcriptionally activated genes, playing a role in DNA demethylation, chromatin remodeling, and brain-specific gene regulation.

Epigentek’s Hydroxymethyl-Seq service uses a proprietary and accurate enzymatic and chemical based method to detect true DNA hydroxmethylation at CpG sites across the entire genome at a single base resolution.

Service #S-2HMS
Proprietary enzymatic and chemical based method to detect DNA hydroxmethylation at CpG sites across the entire genome at single base resolution.
Proprietary 5-hmC sample treatment
5-hmC NGS library prep
HiSeq 2500 sequencing (single read 50 nts)
Bioinformatics analysis

Sample Requirements

Epigenetic Services Sample Requirements
We currently accept DNA samples for our 5-hmC Sequencing (Hydroxymethyl-Seq) service. Proper sample preparation along with the appropriate quality control methods on your end will allow the greatest chance for assay success. Please follow all guidelines for sample requirements, packaging and labeling, and shipping which are outlined on the Submission Instructions page.

Talk to a Scientist

Want more information on our 5-hmC Sequencing (Hydroxymethyl-Seq) service or interested in customizing your research project? We’re here to help! Get in touch with one of our highly knowledgeable epigenetic services experts by calling toll-free at 1-877-374-4368 or emailing services @ epigentek.com.