Description
Mouse monoclonal antibody raised against a partial recombinant USP34, clone 2E2.

Size
100 µg

Purification
Protein A purified

Immunogen
USP34 (NP_055524, 3296 a.a. ~ 3396 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG2a Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunohistochemistry (IHC): 5 µg/ml
Immunofluorescence: 5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (37.11 KDa).

 

Note: This item was formerly catalog no. ABB-8047 (ABB8047)

Applications

Western Blot	USP34 Monoclonal Antibody, clone 2E2. Western Blot analysis of USP34 expression in A-549.
Immunoprecipitation	Not Determined
ELISA	Detection limit for recombinant GST tagged USP34 is approximately 1 ng/ml as a capture antibody.
Immunohistochemistry (IHC)	Immunoperoxidase of monoclonal antibody to USP34 on formalin-fixed paraffin-embedded human seminoma. [Antibody Concentration: 3 µg/ml]
Immunofluorescence	Immunofluorescence of monoclonal antibody to USP34 on HeLa cell. [Antibody Concentration: 10 µg/ml]

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Bonuccelli, G. et. al. (May 2009). Caveolin-1 (P132L), a common breast cancer mutation, confers mammary cell invasiveness and defines a novel stem cell/metastasis-associated gene signature. Am J Pathol. 174(5): 1650-62. PubMed Abstract