Description
Mouse monoclonal antibody raised against a full length recombinant UBE2M, clone 3C4.

Size
100 µg

Purification
Protein A purified

Immunogen
UBE2M (AAH58924, 1 a.a. ~ 184 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG1 Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunofluorescence: 5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (46.24 KDa).

 

Note: This item was formerly catalog no. ABB-8015 (ABB8015)

Applications

Western Blot	UBE2M Monoclonal Antibody, clone 3C4. Western Blot analysis of UBE2M expression in Jurkat.
Immunoprecipitation	Not determined
ELISA	Detection limit for recombinant GST tagged UBE2M is approximately 0.1 ng/ml as a capture antibody.
Immunohistochemistry (IHC)	Not determined
Immunofluorescence	Immunofluorescence of monoclonal antibody to UBE2M on HeLa cell. [Antibody Concentration: 10 µg/ml]

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Miller, B. W. et. al. (October 2009). Application of an integrated physical and functional screening approach to identify inhibitors of the Wnt pathway. Molecular Systems Biology. 5: 315. PubMed Abstract