Description
Mouse monoclonal antibody raised against a partial recombinant UBE2C, clone 9D3.

Size
100 µg

Purification
Protein A purified

Immunogen
UBE2C (NP_008950, 70 a.a. ~ 180 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG2a Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunohistochemistry (IHC): 2.5 µg/ml
Immunofluorescence: 5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (38.21 KDa).

 

 

Note: This item was formerly catalog no. ABB-8012 (ABB8012)

Applications

Western Blot	UBE2C Monoclonal Antibody, clone 9D3. Western Blot analysis of UBE2C expression in HeLa.   Western Blot analysis of UBE2C expression in transfected 293T cell line by UBE2C Monoclonal Antibody, clone 9D3. Lane 1: UBE2C transfected lysate (19.7 KDa). Lane 2: Non-transfected lysate.
Immunoprecipitation	Not determined
ELISA	Detection limit for recombinant GST tagged UBE2C is approximately 0.03 ng/ml as a capture antibody.
Immunohistochemistry (IHC)	Immunoperoxidase of monoclonal antibody to UBE2C on formalin-fixed paraffin-embedded human placenta. [Antibody Concentration: 3 µg/ml]
Immunofluorescence	Immunofluorescence of monoclonal antibody to UBE2C on HeLa cell. [Antibody Concentration 10 µg/ml]

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Chen, S. et. al. (March 2010). Association of clinicopathological features with UbcH10 expression in colorectal cancer. J Cancer Res Clin Oncol. 136(3): 419-26. PubMed Abstract

Jiang, L. et. al. (March 2008). Expression of ubiquitin-conjugating enzyme E2C/UbcH10 in astrocytic tumors. Brain Res. 1201: 161-6. PubMed Abstract