Description
Mouse monoclonal antibody raised against a partial recombinant TRIM28, clone 4E6.

Alternative Names
FLJ29029, KAP1, RNF96, TF1B, TIF1B

Size
100 µg

Purification
Protein A purified

Immunogen
TRIM28 (AAH04978, 379 a.a. ~ 524 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human, Mouse, Rat

Isotype
IgG2b Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
Western Blot: 0.5-1 μg/ml
ELISA: 0.25-1 µg/ml
Immunohistochemistry (IHC): 2.5 µg/ml
Immunofluorescence: 5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (42.06 KDa).

 

Note: This item was formerly catalog no. ABB-1019 (ABB1019) 

Applications

Western Blot	TRIM28 Monoclonal Antibody, clone 4E6. Western Blot analysis in cell lysate of TRIM28 expression in PC-12. TRIM28 monoclonal antibody, clone 4E6. Western Blot analysis in cell lysate of TRIM28 expression in Hela NE. TRIM28 monoclonal antibody, clone 4E6. Western Blot analysis in cell lysate of TRIM28 expression in NIH/3T3. Western Blot analysis of TRIM28 expression in transfected 293T cell line by TRIM28 monoclonal antibody, clone 4E6. Lane 1: TRIM28 transfected lysate (88.5 KDa). Lane 2: Non-transfected lysate.
Immunoprecipitation	Not Determined
ELISA	Applicable
Immunohistochemistry (IHC)	Immunoperoxidase of monoclonal antibody to TRIM28 on formalin-fixed paraffin-embedded human small Intestine. [Antibody Concentration: 3 µg/ml]
Immunofluorescence	Immunofluorescence of monoclonal antibody to TRIM28 on HeLa cell. [Antibody Concentration: 10 µg/ml]

 

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.