Description
Mouse monoclonal antibody raised against a partial recombinant SMURF1, clone 1D7.

Size
100 µg

Purification
Protein A purified

Immunogen
SMURF1 (NP_065162, 165 a.a. ~ 269 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human, Mouse

Isotype
IgG2a Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunohistochemistry (IHC): 5 µg/ml
Immunofluorescence: 5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (37.55 KDa).

 

Note: This item was formerly catalog no. ABB-8033 (ABB8033)

Applications

Western Blot	SMURF1 Monoclonal Antibody, clone 1D7. Western Blot analysis of SMURF1 expression in HeLa. SMURF1 Monoclonal Antibody, clone 1D7. Western Blot analysis of SMURF1 expression in Raw 264.7. SMURF1 Monoclonal Antibody, clone 1D7. Western Blot analysis of SMURF1 expression in NIH/3T3. Western Blot analysis of SMURF1 expression in transfected 293T cell line by SMURF1 Monoclonal Antibody, clone 1D7. Lane 1: SMURF1 transfected lysate (83.4 KDa). Lane 2: Non-transfected lysate.
Immunoprecipitation	Not Determined
ELISA	Detection limit for recombinant GST tagged SMURF1 is approximately 0.1 ng/ml as a capture antibody.
Immunohistochemistry (IHC)	Immunoperoxidase of monoclonal antibody to SMURF1 on formalin-fixed paraffin-embedded human stomach. [Antibody Concentration: 2 µg/ml]
Immunofluorescence	Immunofluorescence of monoclonal antibody to SMURF1 on HeLa cell. [Antibody Concentration: 10 µg/ml]

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Murakami, K. et. al. (July 2010). Smurf1 ubiquitin ligase causes downregulation of BMP receptors and is induced in monocrotaline and hypoxia models of pulmonary arterial hypertension. Exp Biol Med (Maywood). 235(7): 805-13. PubMed Abstract