Description
Mouse monoclonal antibody raised against a partial recombinant SMG1, clone 1A8.

Size
100 µg

Purification
Protein A purified

Immunogen
SMG1 (-, 2922 a.a. ~ 3032 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG Mix Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunohistochemistry (IHC): 2.5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (38.21 KDa).

 

Note: This item was formerly catalog no. ABB-5010 (ABB5010)

Applications

Western Blot	SMG1 Monoclonal Antibody, clone 1A8. Western Blot analysis of SMG1 expression in HeLa NE.
Immunoprecipitation	Not determined
ELISA	Detection limit for recombinant GST tagged SMG1 is approximately 1 ng/ml as a capture antibody.
Immunohistochemistry (IHC)	Immunoperoxidase of monoclonal antibody to SMG1 on formalin-fixed paraffin-embedded in human testis. [Antibody Concentration: 3 µg/ml]
Immunofluorescence	Not determined

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Stalder, L. et. al. (July 2009). Processing bodies are not required for mammalian nonsense-mediated mRNA decay. RNA. 15(7): 1265-73. PubMed Abstract